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KMID : 0380020110260020172
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 2 p.172 ~ p.176
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Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract
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Kim Nam-Young
Jang Hye-Ji
Lee Dong-Geun
Jang Min-Kyung
Lee Seung-Woo
Jeon Myung-Je
Kim Mi-Hyang
Kim Sung-Gu
Lee Sang-Hyeon
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Abstract
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This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 μg/mL of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 μg/mL, respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.
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KEYWORD
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inducible nitric oxide synthase (iNOS), luciferase assay, pine needles, RAW 264.7 cells
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